Generator

Part:BBa_K098010:Design

Designed by: Harvard iGEM 08   Group: iGEM08_Harvard   (2008-10-26)


HO-pcyA, low expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

obtained via PCR


Source

pPCB-AmpR plasmid from Jeff Tabor (?)

References